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CapitalBio Self Positioning Cell-Network Electrophysiological Monitoring System (CEMS) is a revolutionary biochip-based microelectronic sensor system for monitoring the electrophysiological signals of cells. Dozens of microelectrods either for cell positioning or for signal testing are arrayed on the surface of a 10 mm × 10 mm glass slide. Cells can be positioned over recording microelectrodes and cell membrane potential discharges of attached cells can then be recorded automatically by the microelectrodes. Changes to the membrane potentials of the cells are measured and recorded automatically in real time by CEMS. Cultured cells growing in the micro-well are monitored in their natural state without any stimulation undesirable mechanical, electrical or chemical stimulation. The entire process is non-invasive and harmless to the cells.
◆ Non invasive - No mechanical, electrical of chemical stimulation to interfere with cell growth or assay
◆ Automated and real time measurement - Data are collected automatically and analyzed in real time throughout the entire experiment, superior to "end point" assays
◆ Flexible - Unique design allows customization of assay protocols, increases laboratory productivity
◆ Label free - No expensive labels or reporters required
◆ Compatible with downstream genomic analysis - Genome Expression Profiling, Transcription Factor Profiling, SNP Detection Tissue Status
Cellular communication and cellular membrane potential are of fundamental to the development and organization in multicellular organisms. Aberrant cellular communication and membrane potentials contribute to many diseases, such as arrhythmias in the heart, myotonias, peripheral neuropathy, Parkinson′s Disease, Alzheimer′s disease, etc. The extra-cellular signals, such as hormones, neurotransmitters, chemicals and drugs, first reach cellular membrane receptors, which further relay the signal into target compartments inside the cell. Many of the receptor molecules are coupled with ion channels, and regulating channel opening or closing and leading to the cell membrane potential changes.
Function | Na+ channel | K+ channel | Ca++ channel |
Blockers | Tetrodotoxin | Tetraethylammonium | Verapamil |
Saxitoxin | Charybtotoxin | Diltiazem | |
Chlorpromazine | 4-Aminopyridine | alpha-conotoxin | |
Local anesthetics | Local anesthetics | SDZ(-)202791 | |
Activators | Batrachotoxin | Pinacidil | SDZ(-)202791 |
Veratridine | BayK 8644 |
◆ Neuron cell electrophysiology and neuron network communication investigations
◆ Drug effects on neuron cell electrophysiology and neuron network communication
◆ Heart muscle cell electrophysiology research
◆ Drug effects on heart muscle cell electrophysiology
Synaptic transmission | Neurotransmitter | Receptor | Antagonist | Antagonist Effect |
Inhibitory | GABA | GABA-A | BIC | Disinhibition |
Glycine | Glycine | STR | Disinhibition | |
Excitory | Glutamate | Non-NMDA | CNQX | Inhibition |
NMDA | APV | Inhibition |
GABA: γ-aminobutyric acid
NMDA: N-methyl-D-aspartic acid
BIC: bicuculline
STR: strychnine
CNQX: 6-cyano-7-nitroquinoxaline-2,3-dione
APV: 2-amino-5-phosphonovalerate
Cell Network Monitoring Chip Real Time |
Cell Network Monitoring Chip Report |
| Multi-channel real time monitoring graph after drug injection | Change of cellular electrical signal frequency after drug injection |
Cat.No. | Product Name |
409010 | Cell-Network Electrophysiological Monitoring Chip |
