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CapitalBio catalog expression profiling arrays consist of various high quality cDNA and Oligo arrays, such as human, mouse, rice, cotton, silkworm genomic expression arrays, and some specific classes of functional expression arrays. These preprinted arrays have been widely used in life science research for genotyping or gene expression detection of target biological samples. Genomic array studies permit total analysis of cell genomic expression profiles, discovery of differential genes or differentially expressed genes, and discovery of cell phenotype and drug-effect related genes. Classified functional genomic arrays are useful for repeated and detailed experimental analysis for specific functionally-related genes.
Sensitivity | Specificity | Accuracy | |
cDNA arrays | ++++ | ++ | +++① |
Oligo (50-70 mer) arrays | +++ | ++++ | ++++ |
Oligo (25-49 mer) arrays | ++ | ++++ | ++++ |
①: Due to the complexity of cDNA library preparation, which includes large amount of plasmid amplification, PCR、and purification, 30% or more blank spots and mis-printed spots may be produced.
The blank array slides are freshly made in-house with various coatings, under stringent quality controls.
Item | Specification |
Spot size | 120±10 μm |
Absolute coordinates of first spot on slide | X= ±0.1 mm, Y= ±0.1 mm |
Space between subarrays | ±30 μm |
Subarray rotation | 0±0.2 degrees |
Row spacing within a subarray | ±10 μm |
Column spacing within a subarray | ±10 μm |
Spots coordinates within a subarray | ±10 μm shift from a perfect grid with (to be specified) pitch in X and Y directions |
Space between array copies | ±30 μm |
CapitalBio catalog high-density oligonucleotide microarrays consist of 70mers with sequences designed and tested for maximum sensitivity and specificity, while minimizing background and non-specific hybridization. Each probe is arrayed and covalently linked to the slides using optimum glass surface chemistry.
◆ Highly sensitive - comparable to cDNA probes without the drawbacks of PCR amplification
◆ Excellent specificity - sequence optimized to minimize cross-hybridization, allowing analysis of overlapping and homologous genes
◆ Eukaryotic probes biased toward the 3´ end of each gene
◆ Normalized hybridization conditions - melting-temperature (Tm) normalized oligo sets
◆ Reliable and reproducible results
Cat. No. | Product Name | Gene Number |
220010 | Human genome oligo array V1.0 | 22,000 |
220020 | Human genome oligo array V2.0 | 35,000 |
220110 | Human signal transduction oligo array | 896 |
220120 | Human cytokine and inflammatory response oligo array | 495 |
220140 | Human apoptosis oligo array | 367 |
220150 | Human neuron gene oligo array | 198 |
220180 | Human cell cycle oligo array | 100 |
220190 | Human DNA damage and repair oligo array | 101 |
220210 | Human cancer related gene oligo array | 2,747 |
221011 | Mouse genome oligo array | 32,000 |
221110 | Rat genome oligo array V1.0 | 5,700 |
221120 | Rat genome oligo array V2.0 | 27,000 |
221210 | Silkworm genome oligo array | 23,000 |
221410 | Arabidopsis genome oligo array | 29,000 |
221510 | E.coli genome oligo array | 9,300 |
221610 | Drosophila genome oligo array | 15,000 |
221710 | Dog genome oligo array | 25,000 |
221810 | Zebrafish genome oligo array | 30,000 |
221910 | Rhesus Monkey genome oligo array | 35,000 |
222010 | Saccharomyces cerevisiae genome oligo array | 6,400 |
224110 | Cotton genome cDNA array service V1.0 | 12,000 |
224111 | Cotton genome cDNA array service V2.0 | 29,000 |