Life Science

Expression Profiling Arrays

CapitalBio catalog expression profiling arrays consist of various high quality cDNA and Oligo arrays, such as human, mouse, rice, cotton, silkworm genomic expression arrays, and some specific classes of functional expression arrays. These preprinted arrays have been widely used in life science research for genotyping or gene expression detection of target biological samples. Genomic array studies permit total analysis of cell genomic expression profiles, discovery of differential genes or differentially expressed genes, and discovery of cell phenotype and drug-effect related genes. Classified functional genomic arrays are useful for repeated and detailed experimental analysis for specific functionally-related genes.

Overview| Ordering Information|

Characteristics

 
Sensitivity
Specificity
Accuracy
cDNA arrays
++++
++
+++
Oligo (50-70 mer) arrays
+++
++++
++++
Oligo (25-49 mer) arrays
++
++++
++++

①: Due to the complexity of cDNA library preparation, which includes large amount of plasmid amplification, PCR、and purification, 30% or more blank spots and mis-printed spots may be produced.

High Quality Array Production

The blank array slides are freshly made in-house with various coatings, under stringent quality controls.

 

20060222003

 

20060222004

Item
Specification
Spot size
120±10 μm
Absolute coordinates of first spot on slide
X= ±0.1 mm, Y= ±0.1 mm
Space between subarrays
±30 μm
Subarray rotation
0±0.2 degrees
Row spacing within a subarray
±10 μm
Column spacing within a subarray
±10 μm
Spots coordinates within a subarray
±10 μm shift from a perfect grid with (to be specified) pitch in X and Y directions
Space between array copies
±30 μm

Oligonucleotide Microarrays

CapitalBio catalog high-density oligonucleotide microarrays consist of 70mers with sequences designed and tested for maximum sensitivity and specificity, while minimizing background and non-specific hybridization. Each probe is arrayed and covalently linked to the slides using optimum glass surface chemistry.
◆ Highly sensitive - comparable to cDNA probes without the drawbacks of PCR amplification
◆ Excellent specificity - sequence optimized to minimize cross-hybridization, allowing analysis of overlapping and homologous genes
◆ Eukaryotic probes biased toward the 3´ end of each gene
◆ Normalized hybridization conditions - melting-temperature (Tm) normalized oligo sets
◆ Reliable and reproducible results

 

Cat. No.
Product Name
Gene Number
220010
Human genome oligo array V1.0
22,000
220020
Human genome oligo array V2.0
35,000
220110
Human signal transduction oligo array
896
220120
Human cytokine and inflammatory response oligo array
495
220140
Human apoptosis oligo array
367
220150
Human neuron gene oligo array
198
220180
Human cell cycle oligo array
100
220190
Human DNA damage and repair oligo array
101
220210
Human cancer related gene oligo array
2,747
221011
Mouse genome oligo array
32,000
221110
Rat genome oligo array V1.0
5,700
221120
Rat genome oligo array V2.0
27,000
221210
Silkworm genome oligo array
23,000
221410
Arabidopsis genome oligo array
29,000
221510
E.coli genome oligo array
9,300
221610
Drosophila genome oligo array
15,000
221710
Dog genome oligo array
25,000
221810
Zebrafish genome oligo array
30,000
221910
Rhesus Monkey genome oligo array
35,000
222010
Saccharomyces cerevisiae genome oligo array
6,400
224110
Cotton genome cDNA array service V1.0
12,000
224111
Cotton genome cDNA array service V2.0
29,000