Transcription factors (TF) regulate gene transcription through DNA-protein interactions, such as direct binding with DNA. In order to mediate a specific gene transcription, TFs bind in a sequence-specific manner to certain promoter sequences that are usually located upstream from its target gene. By combining chromatin immunoprecipitation using TF specific antibodies, also known as the ChIP assay, then labeling IP-captured promoter-DNA as a probe for hybridization with an array that is pre-spotted with various promoter sequences, it is possible to determine many genetic targets for a given TF. The first generation technology called "ChIP-on-chip" has demonstrated its effectiveness (Ren, B. et al., (2000) Science 290:2306-2309).
Selected by NIH ENCODE project, and invented by UCSD researchers, ChIP-DSL is a new generation of ChIP-Chip technology with greatly increased sensitivity and specificity. It provides a complete product solution for the discovery of virtually all promoters that bind a specific transcription factor. Aviva Systems Biology is pleased to introduce its new ChIP-DSL system.
Transcription factors (TFs) are proteins that bind to promoter regions of genes to regulate their levels of expression.
One transcription factor may regulate hundreds of genes, and one gene′s expression is regulated by multiple transcription factors. Defects in transcription factor regulation, structure and/or function have been implicated in human diseases such as cancer and inflammation, and numerous transcription factors are drug targets. The ability to screen for transcription factor activation is important to drug discovery as well as gene regulation studies.
Traditional methods for measuring active transcription factors include gel-shift or electrophoretic mobility shift assays (EMSA) and colorimetric ELISA-based assays. EMSAs are cumbersome, may involve radioactivity and are not amenable to high-throughput applications. CapitalBio developed an Oligonucleotide Array-based Transcription Factor Assay (OATFA) to profile transcription factor binding activities in human, mouse and yeast cell lysates. This microarray transcription factor profiling system enables researchers to rapidly identify transcription factors that drive biological responses. It employs powerful technologies for simultaneously measuring the binding of transcription factors to their specific regulatory DNA elements.
Transcription regulation in eukaryotes is known to occur through the coordinated action of multiple transcription factors. Understanding the mechanisms, cooperative and synergetic, behind this precise control is important in unrevealing underlying mechanisms of cell differentiation, organism development and the effect of many diseases. Currently, our transcription factor profiling in combination with our expression profiling microarray and promoter array services, provides a powerful platform for researchers to study transcription regulation in eukaryotes. The revolutionary multiplexed profiling technologies can dramatically accelerate studies in fields ranging from basic research in signal transduction and proteomics to target identification, validation, compound screening, toxicity testing, and disease characterization.
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